Facts About attune flow cytometer Revealed
Facts About attune flow cytometer Revealed
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Info-enriched characterization of mobile populations. Morphological facts from illustrations or photos can include to your richness of flow cytometry information. By way of example, the figure exhibits an if not standard apoptosis assay employing Annexin V and PI, introducing mobile imaging to characterize cells in Just about every populace to reveal morphologically distinctive capabilities. These insights couldn't are actually obtained from multiplex staining on your own.
As samples are acquired about the Attune CytPix Flow Cytometer, the high-speed brightfield digicam captures and stores pictures of detected functions at a amount as much as 6,000 visuals per next, depending upon the flow charge and graphic dimension. For higher overall flexibility, Attune Cytometric Software package allows you to adjust the image capture frequency as desired and select a certain gate for image seize, if demanded.
Blend acoustic focusing technological know-how with large-parameter Evaluation for versatile spectral unmixing and standard workflows.
The award-winning Invitrogen Attune CytPix Flow Cytometer combines acoustic focusing using a higher-speed brightfield camera for simultaneous superior throughput flow cytometry and substantial resolution brightfield imaging.
"A picture is not very well worth a thousand dots, but it really genuinely really helps to see visuals. It is great to find out images that correlate with staining. By way of example, AnnexinV-beneficial cells which can be morphologically diverse than balanced cells, or CD14+ monocytes that happen to be more substantial and a lot more textured than CD3+/CD4+/CD14- lymphocytes.
Additionally, it is actually illuminating to determine the amount of doublets slip as a result of traditional singlet gates, and wonderful to verify the position of unpredicted double-optimistic situations in my analysis."
Pair your Attune flow cytometer with the autosampler for greater workflow efficiency, and include robotic integration for optimum throughput.
Dependable picture top quality even at large throughput Acoustic focusing along with a higher-speed camera combine to picture these T cells consistently at very low or significant flow premiums. Effortlessly adjust emphasis and camera options to fulfill experimental prerequisites.
Early signs of this contamination experienced at first been dismissed as debris. Backgating to find new populations. Backgating imaged cells on the Attune CytPix Flow Cytometer also enables you to use morphological features to find out intriguing subpopulations that may not be clear from flow cytometry knowledge by yourself.
Users can perspective party pictures in genuine-time to substantiate sample quality, optimize protocols, and file the morphology of each cell populace identified for analysis. Imaging seize also Advantages from acoustic concentrating, which helps you to position the cells to make sure that a sharp, centered graphic is obtained.
Audio waves empower specific cell alignment, causing Remarkable facts top quality and ~10X more rapidly sample flow costs.
We Earlier shown the strength of imaging CAR-T/Ramos mobile interactions. Allow’s have a look at just the populace of biggest desire, the double constructive activities, To find out more. We could now use prolonged morphological parameters (here, circularity and skewness of intensity) to further more study the features of such populations and refine gating on these situations, escalating details robustness.
Designed-in dimensionality reduction instruments elevated resolution into populations. Fluorescently gated populations back again-gated on to UMAP plot to verify expected inhabitants resolution in lysed total human blood. User can perspective activities from each cluster for Visible confirmation of cell phenotypes.
Adaptable payment approach from Agilent Monetary Methods permit you to receive the important instrumentation you need for fast job, or to develop your lab.
Gating on these extended parameters lets you check here speedily and properly detect populations of interest and ensure gating approach with little or no guide evaluation. The Attune program features entry to state-of-the-art machine Studying analysis resources inside an expertly created person interface, which makes it extremely simple to use.
Because the mobile line experienced Formerly been analyzed on the Attune CytPix Flow Cytometer, the scientists went back again to the pictures and had been in the position to document the microbial infection a minimum of five days earlier.
The Attune CytPix Flow Cytometer lets customers to visualize samples during acquisition, validate the accuracy of gating tactics, and assistance outcomes with trusted documentation for publication purposes.
What seemed to be a homogeneous population is in fact more varied—an Perception that should be viewed as when interpreting outcomes.